| Jared Padalecki | Sam Winchester |
| Jensen Ackles | Dean Winchester |
| Mitch Pileggi | Samuel Campbell /
Azazel |
| Misha Collins | Castiel |
| Genevieve Padalecki | Ruby
(as Genevieve Cortese) |
| Matt Cohen | Young John Winchester
(as Matthew Cohen) |
| Amy Gumenick | Young Mary Campbell |
| Allison Hossack | Deanna Campbell |
| Christopher B. MacCabe | Dr. Brown /
Azazel
(as Christopher MacCabe) |
| Ken Camroux-Taylor | Mr. D |
| Troy Anthony Young | Daniel Elkins |
| Andy Nez | Cop |
| Mark McConchie | Used Car Salesman |
| Nadine Wright | Beth Whitshire |
| Max Lloyd-Jones | Charlie Whitshire |
| Viviana Cruise | Liddy Walsh
(as Viviana Dal Cengio) |
| Robert Dayton | Reg |