| Mark Harmon | Leroy Jethro Gibbs |
| Michael Weatherly | Anthony DiNozzo |
| Cote de Pablo | Ziva David |
| Pauley Perrette | Abby Sciuto |
| Sean Murray | Timothy McGee |
| Rocky Carroll | Leon Vance |
| David McCallum | Donald Mallard |
| Brian Dietzen | Jimmy Palmer |
| Sarah Jane Morris | NCIS Special Agent Erica Jane 'EJ' Barrett |
| Wendy Makkena | Dr. Rachel Cranston |
| Philip Casnoff | Sean Latham |
| Matthew Willig | NCIS Special Agent Simon Cade |
| Scott Wolf | Casey Stratton |
| Matt Craven | Secretary of the Navy Clayton Jarvis |
| Bryan Friday | Captain Felix Wright |
| Alimi Ballard | NCIS Special Agent Gayne Levin |
| Alan Burghard | Range Master |