| Mark Harmon | Leroy Jethro Gibbs |
| Sean Murray | Timothy McGee |
| Wilmer Valderrama | Nick Torres |
| Emily Wickersham | Eleanor Bishop |
| Brian Dietzen | Jimmy Palmer |
| Diona Reasonover | Kasie Hines |
| Rocky Carroll | Leon Vance |
| David McCallum | Donald Mallard |
| William Allen Young | U.S. Secretary of Defense Moses McClaine |
| Victoria Gabrielle Platt | NCIS Special Agent Veronica Tyler |
| Zane Holtz | NCIS Special Agent Dale Sawyer |
| Andrew Pifko | Miles Thatcher |
| Daniel Peera | Nasser Al-Kamal |
| Connie Jackson | Elaine |
| Pam Dawber | Marcie Warren |
| Paolo Andino | Arthur |
| Preston Butler III | Kendle Spotnitz |
| Kamel Haddad | Yemeni Foreign Affairs Minister |
| Brick Jackson | Little Pinky |