| Ben McKenzie | James Gordon |
| Donal Logue | Harvey Bullock |
| David Mazouz | Bruce Wayne |
| Morena Baccarin | Leslie Thompkins |
| Sean Pertwee | Alfred Pennyworth |
| Robin Lord Taylor | Oswald Cobblepot |
| Erin Richards | Barbara Kean |
| Camren Bicondova | Selina Kyle |
| Cory Michael Smith | Edward Nygma |
| Jessica Lucas | Tabitha Galavan
(credit only) |
| Chris Chalk | Lucius Fox |
| Drew Powell | Butch Gilzean
(credit only) |
| Maggie Geha | Ivy Pepper
(credit only) |
| Benedict Samuel | Jervis Tetch |
| Michael Chiklis | Nathaniel Barnes |
| James Carpinello | Mario Falcone |
| Leslie Hendrix | Kathryn |
| Chelsea Spack | Isabella |
| Happy Anderson | Deever Tweed |
| Naian González Norvind | Alice Tetch
(as Naian Gonzalez Norvind) |
| Michael Park | Peter Gordon |
| Jamie Chung | Valerie Vale |
| Adam Petchel | Dumfree Tweed |
| Julian Gavilanes | Craig Sullivan |
| Alejandro Hernandez | Herbalist |
| Lawton Denis | Frankie |
| Juliana Nicole Pinho | Julie
(as Juliana Pinho) |
| Zuleyma Guevara | Nurse |
| Mohammed J. Ali | Lab Technician |
| Donnie Kehr | Man |
| Frank Modica | GCPD Detective
(uncredited) |
| Stuart Schnitzer | Desk Sergeant
(uncredited) |
| Kelly P. Williams | Gotham City Police
(uncredited) |