| Anna Torv | Olivia Dunham |
| Joshua Jackson | Peter Bishop |
| Lance Reddick | Phillip Broyles |
| Kirk Acevedo | Charlie Francis |
| Blair Brown | Nina Sharp
(credit only) |
| Jasika Nicole | Astrid Farnsworth |
| John Noble | Dr. Walter Bishop |
| Ari Graynor | Rachel |
| David Pittu | Robert Swift |
| Keith Nobbs | Carl Bussler |
| Kiersten Warren | Sonia |
| Tim Gallin | Cameron Deglmann |
| Robyn Rikoon | MIT Girl #1 |
| Arjun Gupta | MIT Guy #1 |
| Kevin Rogers | MIT Guy #2 |
| Rafi Silver | Jonathan Swift |
| Ana Berry | Newscaster |
| Craig Alan Edwards | Matt |
| Chris Jackson | EMT #1 |
| Robyn Payne | ND FBI Agent #1 |
| Laura Mann | Receptionist
(as Laura Perloe) |
| Lily Pilblad | Ella |
| Marnie Schulenburg | Mom |
| Jeremy Zorek | Tucker |
| Michael Cerveris | The Observer
(uncredited) |
| Jesse Kazemek | Animal Control
(uncredited) |