| Anna Torv | Olivia Dunham |
| Joshua Jackson | Peter Bishop |
| Lance Reddick | Phillip Broyles |
| Kirk Acevedo | Charlie Francis |
| Blair Brown | Nina Sharp
(credit only) |
| Jasika Nicole | Astrid Farnsworth |
| Mark Valley | John Scott
(credit only) |
| John Noble | Dr. Walter Bishop |
| Billy Burke | Lucas Vogel |
| Jared Harris | David Robert Jones |
| Kenneth Tigar | Warden Johan Lennox |
| Trini Alvarado | Samantha Loeb |
| Chance Kelly | Mitchell Loeb |
| Lars Gerhard | Prison Guard |
| Paul Urcioli | Doctor #1 |
| Leslie Eva Glaser | Nurse #1 |
| Cindy Cheung | Nurse #2 |
| Guiesseppe Jones | FBI Agent |
| John Boyd | Ian Spencer
(uncredited) |
| Michael Cerveris | The Observer
(uncredited) |
| Casey Cipriani | Traveler
(uncredited) |
| Paul Jude Letersky | German Airline Pilot
(uncredited) |
| Ian Mclaughlin | Joseph Smith
(uncredited) |
| Loukas Papas | Biohazard Tech
(uncredited) |
| Darren Ross | German Flight Attendant
(uncredited) |
| Anjuli Sanneman | Traveler
(uncredited) |
| Harry L. Seddon | Mental Patient
(uncredited) |