| Nathan Fillion | Captain Malcolm 'Mal' Reynolds |
| Gina Torres | Zoë Washburne |
| Alan Tudyk | Hoban 'Wash' Washburne |
| Morena Baccarin | Inara Serra |
| Adam Baldwin | Jayne Cobb |
| Jewel Staite | Kaylee Frye |
| Sean Maher | Dr. Simon Tam |
| Summer Glau | River Tam |
| Ron Glass | Shepherd Derrial Book |
| Tom Towles | Lund |
| Andrew Bryniarski | Crow |
| Michael Fairman | Adelai Niska |
| Gregg Henry | Sheriff Bourne |
| Valerie Red-Horse | Deputy |
| Kevin Will | Officer |
| Lina Patel | Ensign |
| David Reynolds | Henchman |
| Jeff Ricketts | Man |
| Dennis Cockrum | Other Man |
| Eric Lange | Fed |
| Tawny René Hamilton | Immigrant Woman #1
(as Tawney René Hamilton) |
| Michelle Ferrara | Immigrant Woman #2 |
| Ernesto Trinidad | Alliance Soldier on Train
(uncredited) |
| Melody White | Bellydancer
(uncredited) |
| Rick Williamson | Bar Guy, Henchman
(uncredited) |