| Joel McHale | Jeff Winger |
| Gillian Jacobs | Britta Perry |
| Danny Pudi | Abed Nadir |
| Yvette Nicole Brown | Shirley Bennett |
| Alison Brie | Annie Edison |
| Donald Glover | Troy Barnes |
| Jim Rash | Dean Pelton |
| Ken Jeong | Ben Chang |
| Jonathan Banks | Professor Buzz Hickey |
| John Oliver | Professor Ian Duncan |
| Richard Erdman | Leonard |
| Charley Koontz | Neil |
| Erik Charles Nielsen | Garrett |
| Ben Folds | Professor Bublitz |
| Danielle Kaplowitz | Vicki |
| Dino Stamatopoulos | Starburns |
| Leslie Simms | Rhonda |
| Derek Houck | Tim Briggs |
| William Rubio | Raul Lopez
(as Will Rubio) |
| Sasha Compère | Gwen Ridley
(as Sasha Compere) |
| Dante Brown | Elijah Bennett |
| Dusan Brown | Jordan Bennett |
| Americo Galli | 'Not This Crack' Student |
| Chad Zapfe | Ass Crack Bandit Impersonator |
| Colin Clive | Dr. Frankenstein
(archive sound) (uncredited) |
| Eddie Lobo | Student
(uncredited) |