| Mark Harmon | Leroy Jethro Gibbs |
| Sean Murray | Timothy McGee |
| Wilmer Valderrama | Nick Torres |
| Emily Wickersham | Ellie Bishop |
| Maria Bello | Jack Sloane |
| Brian Dietzen | Jimmy Palmer |
| Diona Reasonover | Kasie Hines |
| Rocky Carroll | Leon Vance |
| David McCallum | Donald Mallard |
| Dabney Coleman | Army Corporal John Sydney |
| Luke Edwards | Tommy 'Peaches' Mulligan |
| Johnny Wactor | Navy Lieutenant Ross Johnson |
| Sam Littlefield | Oliver Sherry |
| Ian Duncan | Michael Deegan |
| Daniel E. Smith | Cal Frasier |
| Rochelle Robinson | Megan Boone |
| Jin Maley | NCIS Probationary Agent Anna Ventura |
| Jess Allen | Burly Bodyguard |
| Adam Aguirre | Uniformed Room-Service Guy |