| Mark Harmon | Leroy Jethro Gibbs |
| Sean Murray | Timothy McGee |
| Wilmer Valderrama | Nick Torres |
| Emily Wickersham | Ellie Bishop |
| Maria Bello | Jack Sloane |
| Brian Dietzen | Jimmy Palmer |
| Diona Reasonover | Kasie Hines |
| Rocky Carroll | Leon Vance |
| David McCallum | Donald Mallard |
| Pej Vahdat | Nigel Hakim |
| Naomi Grace | Kayla Vance |
| Keon Alexander | Nazy Rickman |
| Robin Karfo | Zaiyema |
| Chad Lindberg | Joey Peanuts |
| James A. Watson Jr. | Navy Vice Admiral Richard Hasley |
| Alexa Hamilton | British Deputy Ambassador Sybil Rigg |
| Jim Meskimen | Doctor Farrel Emerson |
| Lauran September | Emma Thatcher |
| Rob Mainord | Myron Holt |
| Cory Jacob | NCIS Special Agent Rod Beecham |
| Jessica Gardner | Olivia Kahn |
| Danielle Rayne | Marine Colonel Leah Siffler |