| Nathan Fillion | Richard Castle |
| Stana Katic | Kate Beckett |
| Susan Sullivan | Martha Rodgers |
| Ruben Santiago-Hudson | Captain Roy Montgomery |
| Molly C. Quinn | Alexis Castle
(as Molly Quinn) |
| Jon Huertas | Javier Esposito |
| Tamala Jones | Lanie Parish |
| Seamus Dever | Kevin Ryan |
| Bailey Chase | Will Sorenson |
| Ion Overman | Candace Robinson |
| Robert Picardo | Dr. Clark Murray |
| Jackie Geary | Maggie Dowd |
| Robert Costanzo | Sal Tenor |
| Joe Marinelli | Jimmy Moran |
| DeLane Matthews | Jacey Goldberg |
| Kari Coleman | Julia Hammond |
| Carrie Southworth | Courtney Morantz |
| Drew Osborne | Owen |
| Rey Herrera | Mario Guerrera |
| Matt Riedy | Attorney |
| Scott Connors | Agent Winfield |
| Catherine Christensen | Agent Adler |
| Ron Althoff | Heavy
(as Ronnie Althoff) |
| Rachel Leah Cohen | Annoyed Woman
(uncredited) |
| Bruce Holman | Precinct Detective
(uncredited) |
| Gary J. Wayton | Dr. Joshua Leeds
(uncredited) |